resources: directories: root: /XXX/XXX/XXX/XXXX/BulkRNA_BALL_Integration conda_dir: . # if not required set to "." reference: reference data: input-fastq progs: progs results: results qual_check: 01-qcheck trimming: 02-trim alignments: 03-aln counts: 04-counts_bypatient dge: 05-DGE_bypatient_0.1 post: 06-post-analysis_bypatient_0.1 cluster: 07-cluster-analysis_bypatient_0.1 organism: human # choices: human mouse ... genome: GRCh38.primary_assembly.genome.fa # genome_annot: gencode.v34.primary_assembly.gtf # skipped - counts already present design: patient,condition # cov1,cov2,...,condition seq_type: single-end # choices: single-end paired-end # skipped - counts already present strand_type: unstranded # choices: unstranded stranded reverse # skipped - counts already present fdr_thr: 0.1 logfc_thr: 0 rand_walk: 1 # choices: 0 (do not print random walks) 1 (print random walks) post_analysis_tool: DESeq # choices: edgeR DESeq limma fastqfiles: - PT10_395C0H: C0_HIGH_S13 - PT10_395C0L: C0_LOW_S14 - PT10_395C1H: C1_HIGH_S9 - PT10_395C1L: C1_LOW_S10 - PT10_395C3H: C3_HIGH_S11 - PT10_395C3L: C3_LOW_S12 - PT12_27C3R3L: C3_R3_S1 - PT12_27C3R4H: C3_R4_S2 - PT12_27D1R3L: D1_R3_S7 - PT12_27D1R4H: D1_R4_S8 - PT12_27D2R3L: D2_R3_S9 - PT12_27D2R4H: D2_R4_S10 - PT13_37C0R4H: 1_S1_L001,1_S1_L002 - PT13_37C0R5L: 2_S2_L001,2_S2_L002 - PT13_37C1R4H: 3_S3_L001,3_S3_L002 - PT13_37C1R5L: 4_S4_L001,4_S4_L002 #NON USARE TRATTINI samples: - PT10_395C0H: PT10_395,GFP_HIGH - PT10_395C0L: PT10_395,GFP_LOW - PT10_395C1H: PT10_395,GFP_HIGH - PT10_395C1L: PT10_395,GFP_LOW - PT10_395C3H: PT10_395,GFP_HIGH - PT10_395C3L: PT10_395,GFP_LOW - PT12_27C3R3L: PT12_27,GFP_LOW - PT12_27C3R4H: PT12_27,GFP_HIGH - PT12_27D1R3L: PT12_27,GFP_LOW - PT12_27D1R4H: PT12_27,GFP_HIGH - PT12_27D2R3L: PT12_27,GFP_LOW - PT12_27D2R4H: PT12_27,GFP_HIGH - PT13_37C0R4H: PT13_37,GFP_HIGH - PT13_37C0R5L: PT13_37,GFP_LOW - PT13_37C1R4H: PT13_37,GFP_HIGH - PT13_37C1R5L: PT13_37,GFP_LOW contrasts: - GFP_LOW:GFP_HIGH enrichergmtfiles: - c1.all.v7.0.symbols.gmt - c2.all.v7.0.symbols.gmt - c3.all.v7.0.symbols.gmt - c4.all.v7.0.symbols.gmt - c5.all.v7.0.symbols.gmt - c6.all.v7.0.symbols.gmt - c7.all.v7.0.symbols.gmt - h.all.v7.0.symbols.gmt read1_mask: "_R1_001.fastq.gz" read2_mask: "_R2_001.fastq.gz" read_mask: "_R1_001.fastq.gz"