#### Interpretation of T cell states using reference atlas ####
#### Interpretation of T cell states using reference atlas ####
To better characterize the composition of identified CAR-Ts, we adopted **ProjecTILs**, which is a computational method to project a single-cell dataset into a reference atlas to compare them without altering the structure of the reference one. Briefly, CAR-Ts from individual samples were independently mapped on the reference mouse TILs’ atlas; cluster composition was then assessed throughout treatment groups (i.e., Empty, IFN, oIL2, and IFN+oIL2).
To better characterize the composition of identified CAR-Ts, we adopted **ProjecTILs**, which is a computational method to project a single-cell dataset into a reference atlas to compare them without altering the structure of the reference one. Briefly, CAR-Ts from individual samples were independently mapped on the reference mouse TILs’ atlas; cluster composition was then assessed throughout treatment groups (i.e., Empty, IFN, oIL2, and IFN+oIL2).
- ProjectTILS_CART.R: script for the analysis of ProjectTILs on different conditions.
