README.md

# Amodio_Infertility_2023
Amodio G, Giacomini G, Boeri L, et al. **T cell exhaustion and senescence signatures characterize and differentiate infertile men**

### Analyses ###
Single-cell (from 10X Genomics) analysis of CD3+ T cells purified from the peripheral blood of men diagnosed with oligo-astheno-teratozoospermia (OAT, n=4), idiopathic non-obstructive azoospermia (iNOA, n=6), and a control group (FER, n=5).


### Directories and Files ###
- Script: R scripts used for the analyses
  - `1_PreProcessing_Data.R`: Full object creation
  - `2_Infertility_scRNAseq_analysis.R`: 
  - `3_SubsetAnalysis_Annotations.R`: subset analyses of T cells and cluster manual annotation
  - `4_ScoreAnalysis_TcellSubset.R`: 
  - `5_Visualization_Export_Data.R`:
 
 
- Data: results of scRNAseq analysis: