Amodio G, Giacomini G, Boeri L, et al. **T cell exhaustion and senescence signatures characterize and differentiate infertile men**
Amodio G, Giacomini G, Boeri L, et al. **T cell exhaustion and senescence signatures characterize and differentiate infertile men**
### Analyses ###
### Single-Cell RNA Sequencing Analysis ###
Single-cell (from 10X Genomics) analysis of CD3+ T cells purified from the peripheral blood of men diagnosed with oligo-astheno-teratozoospermia (OAT, n=4), idiopathic non-obstructive azoospermia (iNOA, n=6), and a control group (FER, n=5).
**scRNAseq** (from 10X Genomics) analysis of CD3+ T cells purified from the peripheral blood of men diagnosed with oligo-astheno-teratozoospermia (OAT, n=4), idiopathic non-obstructive azoospermia (iNOA, n=6), and a control group (FER, n=5).
scRNAseq analysis was performed using a standard [Seurat](https://satijalab.org/seurat/) pipeline that includes the following steps starting from a minimal object after loading of 10X data to markers identification:
**scRNAseq** analysis was performed using a standard [Seurat](https://satijalab.org/seurat/) pipeline that includes the following steps starting from a minimal object after loading of 10X data to markers identification:
- Preprocessing and cell filtering
- Preprocessing and cell filtering
- Each sample was pre-processed and cells with mitochondrial RNA percentages higher than 10 and a number of features <1200or>6000, were filtered out.
- Each sample was pre-processed and cells with mitochondrial RNA percentages higher than 10 and a number of features <1200or>6000, were filtered out.
