Update README.md

README.md

 # Amodio_Infertility_2023
 Amodio G, Giacomini G, Boeri L, et al. **T cell exhaustion and senescence signatures characterize and differentiate infertile men**
 
+### Analyses ###
+Single-cell (from 10X Genomics) analysis of CD3+ T cells purified from the peripheral blood of men diagnosed with oligo-astheno-teratozoospermia (OAT, n=4), idiopathic non-obstructive azoospermia (iNOA, n=6), and a control group (FER, n=5).
+
 
 ### Directories and Files ###
-- Script: R scripts used for the analyses:
+- Script: R scripts used for the analyses
+  - `1_PreProcessing_Data.R`: Full object creation
+  - `2_Infertility_scRNAseq_analysis.R`: 
+  - `3_SubsetAnalysis_Annotations.R`: subset analyses of T cells and cluster manual annotation
+  - `4_ScoreAnalysis_TcellSubset.R`: 
+  - `5_Visualization_Export_Data.R`:
+ 
+ 
+- Data: results of scRNAseq analysis: 
+
+
 
-- Data: results of scRNAseq analysis: