Update README.md

README.md

-# Birocchi_SciTraMed2022_RNAseq
+# Birocchi_SciTraMed2022_scRNAseq #
 
-RNA-seq data of Science Translational Medicine 2022
\ No newline at end of file
+Birocchi F, Cusimano M, Rossari F, Beretta S et al.
+**Reprogramming of the immune infiltrate by targeted inducible delivery of stimulatory cytokines inhibits growth and promotes survival in a mouse model of glioblastoma multiforme.**
+_Science Translational Medicine_ 2022.
+- GEO: [GSE197761](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE197761)
+
+---
+
+### Analyses ###
+
+Stanrdard RNA-seq pipeline on 18 samples.<br>
+RNA-Seq analysis on sorted glioblastoma cells (GFP+) and unaffected brain hemisphere samples of IFN-a-DHFR gene therapy treated, recombinant IFN-a1 treated, and control mice.<br>
+Differential gene expression analysis on treated samples against control ones, and Gene Set Enrichment Analysis (GSEA) on pre-ranked gene lists (ordered by log2 Fold Change values) agasint the MSigDB Hallmark database.