Commit cdf0a1b7 authored by Stefano Beretta's avatar Stefano Beretta
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Update README.md

parent 7c76b6a3
...@@ -9,7 +9,7 @@ _Nature Biotechnology_ 2023. ...@@ -9,7 +9,7 @@ _Nature Biotechnology_ 2023.
### Analyses ### ### Analyses ###
Sequencing data were analyzed with the [BAR-Seq2](https://bitbucket.org/bereste/bar-seq2) pipeline, with the following parameters: Sequencing data were analyzed with the [_BAR-Seq2_](https://bitbucket.org/bereste/bar-seq2) pipeline, with the following parameters:
- `--trim-sequences --trim-opt '-m 50 -q 30'` to trim low-quality bases and keeping sequences having length >50bp; - `--trim-sequences --trim-opt '-m 50 -q 30'` to trim low-quality bases and keeping sequences having length >50bp;
- `--edit-distance 2`; - `--edit-distance 2`;
- `--min-count 2` to keep only barcodes having minimum count equal to 2. - `--min-count 2` to keep only barcodes having minimum count equal to 2.
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