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Fiumara_BasePrimeEd2022
Fiumara_BasePrimeEd2022_BARseq
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cdf0a1b7
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cdf0a1b7
authored
Jul 25, 2023
by
Stefano Beretta
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Update README.md
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@@ -9,7 +9,7 @@ _Nature Biotechnology_ 2023.
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@@ -9,7 +9,7 @@ _Nature Biotechnology_ 2023.
### Analyses ###
### Analyses ###
Sequencing data were analyzed with the
[
BAR-Seq2
](
https://bitbucket.org/bereste/bar-seq2
)
pipeline, with the following parameters:
Sequencing data were analyzed with the
[
_
BAR-Seq2
_
](
https://bitbucket.org/bereste/bar-seq2
)
pipeline, with the following parameters:
-
`--trim-sequences --trim-opt '-m 50 -q 30'`
to trim low-quality bases and keeping sequences having length >50bp;
-
`--trim-sequences --trim-opt '-m 50 -q 30'`
to trim low-quality bases and keeping sequences having length >50bp;
-
`--edit-distance 2`
;
-
`--edit-distance 2`
;
-
`--min-count 2`
to keep only barcodes having minimum count equal to 2.
-
`--min-count 2`
to keep only barcodes having minimum count equal to 2.
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