Interpretation of T cell states using reference atlas

To better characterize the composition of identified CAR-Ts, we adopted ProjecTILs, which is a computational method to project a single-cell dataset into a reference atlas to compare them without altering the structure of the reference one. Briefly, CAR-Ts from individual samples were independently mapped on the reference mouse TILs’ atlas; cluster composition was then assessed throughout treatment groups (i.e., Empty, IFN, oIL2, and IFN+oIL2).