README.md

# Squadrito_LiverTumor2022_scRNAseq

Kerzel T, Giacca G, Beretta S, Scotti MG, et al.
**Targeted Interferon Delivery Through In Vivo Engineered Macrophages Eradicates Liver Metastases.**
2022.

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### Directories and Files ###
- SampleSheet.csv: names of samples and corresponding treatment condisions
- scripts: folder with R scripts used for the analyses
  - `0_PreProcessData.R`: initial analysis with DoubletFinder and Full object creation;
  - `1_Analysis_TNK.R`: subset of T and NK, and analysis;
  - `2_Analysis_APC.R`: subset of APC and analysis;
  - `3_Analysis_UndRem.R`: integration of subset analyses and _undetermined_ removal;
  - `4_Paper_Results.R`: post-analysis and visualization for paper.
- data: results of the analyses
  - Full_final_*: results of Full object analysis;
  - APCs_cells_*: results of APC analysis;
  - T_NK_cells_*: results of T and NK analysis;
  - scRNAseq_*.xlsx: excel files with markers.

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### Analyses ###

Single-cell (from 10X Genomics) analysis of 8 liver tumoral samples.