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Vavassori_LNP2022
Vavassori_LNP2022_RNAseq_electro
Commits
fec670b4
Commit
fec670b4
authored
Oct 17, 2022
by
Stefano Beretta
Browse files
Added RNAseq config file.
parent
391e802f
Changes
1
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config.yaml
0 → 100644
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fec670b4
resources
:
directories
:
root
:
Genewiz_RNAseq_VavassoriV
conda_dir
:
anaconda3/envs/rnaseq
# if not required set to "."
reference
:
reference
data
:
input-fastq
progs
:
progs
results
:
results
qual_check
:
01-qcheck
trimming
:
02-trim
alignments
:
03-aln
counts
:
04-counts-D4
# D12
dge
:
05-DGE-D4
# D12
post
:
06-post-analysis-D4
# D12
organism
:
human
# choices: human mouse ...
genome
:
GRCh38.p12
genome_annot
:
gencode.v35.primary_assembly.annotation.gtf
design
:
donor,condition
# cov1,cov2,...,condition
seq_type
:
paired-end
# choices: single-end paired-end
strand_type
:
unstranded
# choices: unstranded stranded reverse
fdr_thr
:
0.05
logfc_thr
:
0
rand_walk
:
0
# choices: 0 (do not print random walks) 1 (print random walks)
post_analysis_tool
:
DESeq
# choices: edgeR DESeq
fastqfiles
:
#- Sample1: 1--1
#- Sample2: 2--2
#- Sample3: 3--3
-
Sample4
:
4--4
-
Sample5
:
5--5
-
Sample6
:
6--6
-
Sample7
:
7--7
-
Sample8
:
8--8
-
Sample9
:
9--9
-
Sample10
:
10--10
-
Sample11
:
11--11
-
Sample12
:
12--12
-
Sample13
:
13--13
-
Sample14
:
14--14
-
Sample15
:
15--15
-
Sample16
:
16--16
-
Sample17
:
17--17
-
Sample18
:
18--18
#- Sample19: 19--19
#- Sample20: 20--20
#- Sample21: 21--21
#- Sample22: 22--22
#- Sample23: 23--23
#- Sample24: 24--24
#- Sample25: 25--25
#- Sample26: 26--26
#- Sample27: 27--27
#- Sample28: 28--28
#- Sample29: 29--29
#- Sample30: 30--30
#- Sample31: 31--31
#- Sample32: 32--32
#- Sample33: 33--33
samples
:
#- Sample1: Donor1,UT_D3
#- Sample2: Donor2,UT_D3
#- Sample3: Donor3,UT_D3
-
Sample4
:
Donor1,UT_D4
-
Sample5
:
Donor1,UT_electro_D4
-
Sample6
:
Donor1,RNP_D4
-
Sample7
:
Donor1,RNP_AAV6_D4
-
Sample8
:
Donor1,AAV6_D4
-
Sample9
:
Donor2,UT_D4
-
Sample10
:
Donor2,UT_electro_D4
-
Sample11
:
Donor2,RNP_D4
-
Sample12
:
Donor2,RNP_AAV6_D4
-
Sample13
:
Donor2,AAV6_D4
-
Sample14
:
Donor3,UT_D4
-
Sample15
:
Donor3,UT_electro_D4
-
Sample16
:
Donor3,RNP_D4
-
Sample17
:
Donor3,RNP_AAV6_D4
-
Sample18
:
Donor3,AAV6_D4
#- Sample19: Donor1,UT_D12
#- Sample20: Donor1,UT_electro_D12
#- Sample21: Donor1,RNP_D12
#- Sample22: Donor1,RNP_AAV6_D12
#- Sample23: Donor1,AAV6_D12
#- Sample24: Donor2,UT_D12
#- Sample25: Donor2,UT_electro_D12
#- Sample26: Donor2,RNP_D12
#- Sample27: Donor2,RNP_AAV6_D12
#- Sample28: Donor2,AAV6_D12
#- Sample29: Donor3,UT_D12
#- Sample30: Donor3,UT_electro_D12
#- Sample31: Donor3,RNP_D12
#- Sample32: Donor3,RNP_AAV6_D12
#- Sample33: Donor3,AAV6_D12
contrasts
:
-
UT_electro_D4:UT_D4
-
RNP_D4:UT_electro_D4
-
RNP_D4:UT_D4
-
AAV6_D4:RNP_D4
-
AAV6_D4:UT_electro_D4
-
AAV6_D4:UT_D4
-
RNP_AAV6_D4:AAV6_D4
-
RNP_AAV6_D4:RNP_D4
-
RNP_AAV6_D4:UT_electro_D4
-
RNP_AAV6_D4:UT_D4
-
UT_electro_D4:AAV6_D4
-
RNP_D4:AAV6_D4
#- UT_electro_D12:UT_D12
#- RNP_D12:UT_electro_D12
#- RNP_D12:UT_D12
#- AAV6_D12:RNP_D12
#- AAV6_D12:UT_electro_D12
#- AAV6_D12:UT_D12
#- RNP_AAV6_D12:AAV6_D12
#- RNP_AAV6_D12:RNP_D12
#- RNP_AAV6_D12:UT_electro_D12
#- RNP_AAV6_D12:UT_D12
enrichergmtfiles
:
-
h.all.v7.2.symbols.gmt
read1_mask
:
"
_R1_001.fastq.gz"
read2_mask
:
"
_R2_001.fastq.gz"
read_mask
:
"
_R1_001.fastq.gz"
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