Commit fec670b4 authored by Stefano Beretta's avatar Stefano Beretta
Browse files

Added RNAseq config file.

parent 391e802f
resources:
directories:
root: Genewiz_RNAseq_VavassoriV
conda_dir: anaconda3/envs/rnaseq # if not required set to "."
reference: reference
data: input-fastq
progs: progs
results: results
qual_check: 01-qcheck
trimming: 02-trim
alignments: 03-aln
counts: 04-counts-D4 # D12
dge: 05-DGE-D4 # D12
post: 06-post-analysis-D4 # D12
organism: human # choices: human mouse ...
genome: GRCh38.p12
genome_annot: gencode.v35.primary_assembly.annotation.gtf
design: donor,condition # cov1,cov2,...,condition
seq_type: paired-end # choices: single-end paired-end
strand_type: unstranded # choices: unstranded stranded reverse
fdr_thr: 0.05
logfc_thr: 0
rand_walk: 0 # choices: 0 (do not print random walks) 1 (print random walks)
post_analysis_tool: DESeq # choices: edgeR DESeq
fastqfiles:
#- Sample1: 1--1
#- Sample2: 2--2
#- Sample3: 3--3
- Sample4: 4--4
- Sample5: 5--5
- Sample6: 6--6
- Sample7: 7--7
- Sample8: 8--8
- Sample9: 9--9
- Sample10: 10--10
- Sample11: 11--11
- Sample12: 12--12
- Sample13: 13--13
- Sample14: 14--14
- Sample15: 15--15
- Sample16: 16--16
- Sample17: 17--17
- Sample18: 18--18
#- Sample19: 19--19
#- Sample20: 20--20
#- Sample21: 21--21
#- Sample22: 22--22
#- Sample23: 23--23
#- Sample24: 24--24
#- Sample25: 25--25
#- Sample26: 26--26
#- Sample27: 27--27
#- Sample28: 28--28
#- Sample29: 29--29
#- Sample30: 30--30
#- Sample31: 31--31
#- Sample32: 32--32
#- Sample33: 33--33
samples:
#- Sample1: Donor1,UT_D3
#- Sample2: Donor2,UT_D3
#- Sample3: Donor3,UT_D3
- Sample4: Donor1,UT_D4
- Sample5: Donor1,UT_electro_D4
- Sample6: Donor1,RNP_D4
- Sample7: Donor1,RNP_AAV6_D4
- Sample8: Donor1,AAV6_D4
- Sample9: Donor2,UT_D4
- Sample10: Donor2,UT_electro_D4
- Sample11: Donor2,RNP_D4
- Sample12: Donor2,RNP_AAV6_D4
- Sample13: Donor2,AAV6_D4
- Sample14: Donor3,UT_D4
- Sample15: Donor3,UT_electro_D4
- Sample16: Donor3,RNP_D4
- Sample17: Donor3,RNP_AAV6_D4
- Sample18: Donor3,AAV6_D4
#- Sample19: Donor1,UT_D12
#- Sample20: Donor1,UT_electro_D12
#- Sample21: Donor1,RNP_D12
#- Sample22: Donor1,RNP_AAV6_D12
#- Sample23: Donor1,AAV6_D12
#- Sample24: Donor2,UT_D12
#- Sample25: Donor2,UT_electro_D12
#- Sample26: Donor2,RNP_D12
#- Sample27: Donor2,RNP_AAV6_D12
#- Sample28: Donor2,AAV6_D12
#- Sample29: Donor3,UT_D12
#- Sample30: Donor3,UT_electro_D12
#- Sample31: Donor3,RNP_D12
#- Sample32: Donor3,RNP_AAV6_D12
#- Sample33: Donor3,AAV6_D12
contrasts:
- UT_electro_D4:UT_D4
- RNP_D4:UT_electro_D4
- RNP_D4:UT_D4
- AAV6_D4:RNP_D4
- AAV6_D4:UT_electro_D4
- AAV6_D4:UT_D4
- RNP_AAV6_D4:AAV6_D4
- RNP_AAV6_D4:RNP_D4
- RNP_AAV6_D4:UT_electro_D4
- RNP_AAV6_D4:UT_D4
- UT_electro_D4:AAV6_D4
- RNP_D4:AAV6_D4
#- UT_electro_D12:UT_D12
#- RNP_D12:UT_electro_D12
#- RNP_D12:UT_D12
#- AAV6_D12:RNP_D12
#- AAV6_D12:UT_electro_D12
#- AAV6_D12:UT_D12
#- RNP_AAV6_D12:AAV6_D12
#- RNP_AAV6_D12:RNP_D12
#- RNP_AAV6_D12:UT_electro_D12
#- RNP_AAV6_D12:UT_D12
enrichergmtfiles:
- h.all.v7.2.symbols.gmt
read1_mask: "_R1_001.fastq.gz"
read2_mask: "_R2_001.fastq.gz"
read_mask: "_R1_001.fastq.gz"
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