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Vavassori_LNP2022
Vavassori_LNP2022_RNAseq_lnps
Commits
59ae6cb2
Commit
59ae6cb2
authored
Oct 17, 2022
by
Stefano Beretta
Browse files
Added RNAseq config file.
parent
64feb172
Changes
1
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config.yaml
0 → 100644
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59ae6cb2
resources
:
directories
:
root
:
vavassori_lnp2022_rnaseq_lnps
conda_dir
:
anaconda3/envs/rnaseq
# if not required set to "."
reference
:
reference
data
:
input-fastq
progs
:
rna-seq
results
:
results
qual_check
:
01-qcheck
trimming
:
02-trim
alignments
:
03-aln
counts
:
04-counts-T
# HSPC
dge
:
05-DGE-T
# HSPC
post
:
06-post-analysis-T
# HSPC
organism
:
human
# choices: human mouse ...
genome
:
GRCh38.v35
genome_annot
:
gencode.v35.primary_assembly.annotation.gtf
design
:
donor,condition
# cov1,cov2,...,condition
seq_type
:
paired-end
# choices: single-end paired-end
strand_type
:
unstranded
# choices: unstranded stranded reverse
fdr_thr
:
0.05
logfc_thr
:
0
rand_walk
:
0
# choices: 0 (do not print random walks) 1 (print random walks)
post_analysis_tool
:
DESeq
# choices: edgeR DESeq
fastqfiles
:
#- Sample1: S1
#- Sample2: S2
#- Sample3: S3
#- Sample4: S4
#- Sample5: S5
#- Sample6: S6
#- Sample7: S7
#- Sample8: S8
#- Sample9: S9
#- Sample10: S10
#- Sample11: S11
#- Sample12: S12
#- Sample13: S13
#- Sample14: S14
#- Sample15: S15
#- Sample16: S16
#- Sample17: S17
#- Sample18: S18
-
Sample19
:
S19
-
Sample20
:
S20
-
Sample21
:
S21
-
Sample22
:
S22
-
Sample23
:
S23
-
Sample24
:
S24
-
Sample25
:
S25
-
Sample26
:
S26
-
Sample27
:
S27
-
Sample28
:
S28
-
Sample29
:
S29
-
Sample30
:
S30
-
Sample31
:
S31
-
Sample32
:
S32
-
Sample33
:
S33
-
Sample34
:
S34
-
Sample35
:
S35
-
Sample36
:
S36
samples
:
#- Sample1: HSPC_UT
#- Sample2: HSPC_UTelectro
#- Sample3: HSPC_electroRNP
#- Sample4: HSPC_electroRNA
#- Sample5: HSPC_emptyLNPs
#- Sample6: HSPC_LNPsRNA
#- Sample7: HSPC_UT
#- Sample8: HSPC_UTelectro
#- Sample9: HSPC_electroRNP
#- Sample10: HSPC_electroRNA
#- Sample11: HSPC_emptyLNPs
#- Sample12: HSPC_LNPsRNA
#- Sample13: HSPC_UT
#- Sample14: HSPC_UTelectro
#- Sample15: HSPC_electroRNP
#- Sample16: HSPC_electroRNA
#- Sample17: HSPC_emptyLNPs
#- Sample18: HSPC_LNPsRNA
-
Sample19
:
Donor1,T_UT
-
Sample20
:
Donor1,T_UTelectro
-
Sample21
:
Donor1,T_electroRNP
-
Sample22
:
Donor1,T_electroRNA
-
Sample23
:
Donor1,T_emptyLNPs
-
Sample24
:
Donor1,T_LNPsRNA
-
Sample25
:
Donor2,T_UT
-
Sample26
:
Donor2,T_UTelectro
-
Sample27
:
Donor2,T_electroRNP
-
Sample28
:
Donor2,T_electroRNA
-
Sample29
:
Donor2,T_emptyLNPs
-
Sample30
:
Donor2,T_LNPsRNA
-
Sample31
:
Donor3,T_UT
-
Sample32
:
Donor3,T_UTelectro
-
Sample33
:
Donor3,T_electroRNP
-
Sample34
:
Donor3,T_electroRNA
-
Sample35
:
Donor3,T_emptyLNPs
-
Sample36
:
Donor3,T_LNPsRNA
contrasts
:
# - HSPC_UTelectro:HSPC_UT
# - HSPC_electroRNP:HSPC_UT
# - HSPC_electroRNA:HSPC_UT
# - HSPC_emptyLNPs:HSPC_UT
# - HSPC_LNPsRNA:HSPC_UT
# - HSPC_electroRNP:HSPC_UTelectro
# - HSPC_electroRNA:HSPC_UTelectro
# - HSPC_emptyLNPs:HSPC_UTelectro
# - HSPC_LNPsRNA:HSPC_UTelectro
# - HSPC_electroRNA:HSPC_electroRNP
# - HSPC_emptyLNPs:HSPC_electroRNP
# - HSPC_LNPsRNA:HSPC_electroRNP
# - HSPC_emptyLNPs:HSPC_electroRNA
# - HSPC_LNPsRNA:HSPC_electroRNA
# - HSPC_LNPsRNA:HSPC_emptyLNPs
-
T_UTelectro:T_UT
-
T_electroRNP:T_UT
-
T_electroRNA:T_UT
-
T_emptyLNPs:T_UT
-
T_LNPsRNA:T_UT
-
T_electroRNP:T_UTelectro
-
T_electroRNA:T_UTelectro
-
T_emptyLNPs:T_UTelectro
-
T_LNPsRNA:T_UTelectro
-
T_electroRNA:T_electroRNP
-
T_emptyLNPs:T_electroRNP
-
T_LNPsRNA:T_electroRNP
-
T_emptyLNPs:T_electroRNA
-
T_LNPsRNA:T_electroRNA
-
T_LNPsRNA:T_emptyLNPs
enrichergmtfiles
:
-
h.all.v7.2.symbols.gmt
read1_mask
:
"
_R1_001.fastq.gz"
read2_mask
:
"
_R2_001.fastq.gz"
read_mask
:
"
_R1_001.fastq.gz"
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