Commit 59ae6cb2 authored by Stefano Beretta's avatar Stefano Beretta
Browse files

Added RNAseq config file.

parent 64feb172
resources:
directories:
root: vavassori_lnp2022_rnaseq_lnps
conda_dir: anaconda3/envs/rnaseq # if not required set to "."
reference: reference
data: input-fastq
progs: rna-seq
results: results
qual_check: 01-qcheck
trimming: 02-trim
alignments: 03-aln
counts: 04-counts-T # HSPC
dge: 05-DGE-T # HSPC
post: 06-post-analysis-T # HSPC
organism: human # choices: human mouse ...
genome: GRCh38.v35
genome_annot: gencode.v35.primary_assembly.annotation.gtf
design: donor,condition # cov1,cov2,...,condition
seq_type: paired-end # choices: single-end paired-end
strand_type: unstranded # choices: unstranded stranded reverse
fdr_thr: 0.05
logfc_thr: 0
rand_walk: 0 # choices: 0 (do not print random walks) 1 (print random walks)
post_analysis_tool: DESeq # choices: edgeR DESeq
fastqfiles:
#- Sample1: S1
#- Sample2: S2
#- Sample3: S3
#- Sample4: S4
#- Sample5: S5
#- Sample6: S6
#- Sample7: S7
#- Sample8: S8
#- Sample9: S9
#- Sample10: S10
#- Sample11: S11
#- Sample12: S12
#- Sample13: S13
#- Sample14: S14
#- Sample15: S15
#- Sample16: S16
#- Sample17: S17
#- Sample18: S18
- Sample19: S19
- Sample20: S20
- Sample21: S21
- Sample22: S22
- Sample23: S23
- Sample24: S24
- Sample25: S25
- Sample26: S26
- Sample27: S27
- Sample28: S28
- Sample29: S29
- Sample30: S30
- Sample31: S31
- Sample32: S32
- Sample33: S33
- Sample34: S34
- Sample35: S35
- Sample36: S36
samples:
#- Sample1: HSPC_UT
#- Sample2: HSPC_UTelectro
#- Sample3: HSPC_electroRNP
#- Sample4: HSPC_electroRNA
#- Sample5: HSPC_emptyLNPs
#- Sample6: HSPC_LNPsRNA
#- Sample7: HSPC_UT
#- Sample8: HSPC_UTelectro
#- Sample9: HSPC_electroRNP
#- Sample10: HSPC_electroRNA
#- Sample11: HSPC_emptyLNPs
#- Sample12: HSPC_LNPsRNA
#- Sample13: HSPC_UT
#- Sample14: HSPC_UTelectro
#- Sample15: HSPC_electroRNP
#- Sample16: HSPC_electroRNA
#- Sample17: HSPC_emptyLNPs
#- Sample18: HSPC_LNPsRNA
- Sample19: Donor1,T_UT
- Sample20: Donor1,T_UTelectro
- Sample21: Donor1,T_electroRNP
- Sample22: Donor1,T_electroRNA
- Sample23: Donor1,T_emptyLNPs
- Sample24: Donor1,T_LNPsRNA
- Sample25: Donor2,T_UT
- Sample26: Donor2,T_UTelectro
- Sample27: Donor2,T_electroRNP
- Sample28: Donor2,T_electroRNA
- Sample29: Donor2,T_emptyLNPs
- Sample30: Donor2,T_LNPsRNA
- Sample31: Donor3,T_UT
- Sample32: Donor3,T_UTelectro
- Sample33: Donor3,T_electroRNP
- Sample34: Donor3,T_electroRNA
- Sample35: Donor3,T_emptyLNPs
- Sample36: Donor3,T_LNPsRNA
contrasts:
# - HSPC_UTelectro:HSPC_UT
# - HSPC_electroRNP:HSPC_UT
# - HSPC_electroRNA:HSPC_UT
# - HSPC_emptyLNPs:HSPC_UT
# - HSPC_LNPsRNA:HSPC_UT
# - HSPC_electroRNP:HSPC_UTelectro
# - HSPC_electroRNA:HSPC_UTelectro
# - HSPC_emptyLNPs:HSPC_UTelectro
# - HSPC_LNPsRNA:HSPC_UTelectro
# - HSPC_electroRNA:HSPC_electroRNP
# - HSPC_emptyLNPs:HSPC_electroRNP
# - HSPC_LNPsRNA:HSPC_electroRNP
# - HSPC_emptyLNPs:HSPC_electroRNA
# - HSPC_LNPsRNA:HSPC_electroRNA
# - HSPC_LNPsRNA:HSPC_emptyLNPs
- T_UTelectro:T_UT
- T_electroRNP:T_UT
- T_electroRNA:T_UT
- T_emptyLNPs:T_UT
- T_LNPsRNA:T_UT
- T_electroRNP:T_UTelectro
- T_electroRNA:T_UTelectro
- T_emptyLNPs:T_UTelectro
- T_LNPsRNA:T_UTelectro
- T_electroRNA:T_electroRNP
- T_emptyLNPs:T_electroRNP
- T_LNPsRNA:T_electroRNP
- T_emptyLNPs:T_electroRNA
- T_LNPsRNA:T_electroRNA
- T_LNPsRNA:T_emptyLNPs
enrichergmtfiles:
- h.all.v7.2.symbols.gmt
read1_mask: "_R1_001.fastq.gz"
read2_mask: "_R2_001.fastq.gz"
read_mask: "_R1_001.fastq.gz"
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