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Amodio_Infertility_2023
Commits
584d943d
Commit
584d943d
authored
Jan 08, 2024
by
Giorgia Giacomini
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Update README.md
parent
7016c201
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@@ -9,7 +9,7 @@ scRNAseq analysis was performed using a standard [Seurat](https://satijalab.org/
-
Preprocessing and cell filtering
-
Each sample was pre-processed and cells with mitochondrial RNA percentages higher than 10 and a number of features
<
1200
or
>
6000, were filtered out.
-
Normalization
-
Default Seurat settings
(
[NormalizeData function
]
)(https://satijalab.org/seurat/reference/normalizedata)
-
Default Seurat settings
[
(
NormalizeData function)
]
(
https://satijalab.org/seurat/reference/normalizedata
)
-
Scaling:
-
Data was regressed out by passing UMI count, the percentage of mitochondrial genes, the difference between the cell cycle phases scores.
-
Dimensionality reduction and Harmony batch removal:
...
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