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Commit 584d943d authored by Giorgia Giacomini's avatar Giorgia Giacomini
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Update README.md

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README.md
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...@@ -9,7 +9,7 @@ scRNAseq analysis was performed using a standard [Seurat](https://satijalab.org/ ...@@ -9,7 +9,7 @@ scRNAseq analysis was performed using a standard [Seurat](https://satijalab.org/
- Preprocessing and cell filtering - Preprocessing and cell filtering
- Each sample was pre-processed and cells with mitochondrial RNA percentages higher than 10 and a number of features <1200 or >6000, were filtered out. - Each sample was pre-processed and cells with mitochondrial RNA percentages higher than 10 and a number of features <1200 or >6000, were filtered out.
- Normalization - Normalization
- Default Seurat settings ([NormalizeData function])(https://satijalab.org/seurat/reference/normalizedata) - Default Seurat settings [(NormalizeData function)](https://satijalab.org/seurat/reference/normalizedata)
- Scaling: - Scaling:
- Data was regressed out by passing UMI count, the percentage of mitochondrial genes, the difference between the cell cycle phases scores. - Data was regressed out by passing UMI count, the percentage of mitochondrial genes, the difference between the cell cycle phases scores.
- Dimensionality reduction and Harmony batch removal: - Dimensionality reduction and Harmony batch removal:
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