README.md

# Birocchi_SciTraMed2022_scRNAseq #

Birocchi F, Cusimano M, Rossari F, Beretta S et al.
**Targeted inducible delivery of immunoactivating cytokines reprograms glioblastoma microenvironment and inhibits growth in mouse models.**
_Science Translational Medicine_ 2022.
- PMID: [35857642](https://www.ncbi.nlm.nih.gov/pubmed/35857642)
- DOI: [10.1126/scitranslmed.abl4106](https://doi.org/10.1126/scitranslmed.abl4106)
- GEO: [GSE197761](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE197761)

---

### Analyses ###

Stanrdard RNA-seq pipeline on 18 samples.<br>
RNA-Seq analysis on sorted glioblastoma cells (GFP+) and unaffected brain hemisphere samples of IFN-a-DHFR gene therapy treated, recombinant IFN-a1 treated, and control mice.<br>
Differential gene expression analysis on treated samples against control ones, and Gene Set Enrichment Analysis (GSEA) on pre-ranked gene lists (ordered by log2 Fold Change values) agasint the MSigDB Hallmark database.